HTB-14 ™
Explore our dataU-87 MG is a cell line with epithelial morphology that was isolated from malignant gliomas from a male patient, likely with Glioblastoma. Use these cells in your neuroscience and immuno-oncology research.
- Product category
-
Human cells
- Organism
-
hom*o sapiens, human
- Morphology
- epithelial
- Tissue
- Brain
- Disease
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Likely Glioblastoma
- Applications
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3D cell culture
Neuroscience
- Product format
- Frozen
- Storage conditions
-
Vapor phase of liquid nitrogen
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Required Products
These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.
Documentation
Certificate of Analysis Download
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Certificate of Origin Download
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This product sheet is not available online. We only provide this product sheet to customers who have purchased this biosafety level 3 product. If you purchased this product, please contact Technical Service for this product sheet.
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Please check the Product Sheet and Safety Data Sheet Landing pagefor more information.
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
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Required Products
These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.
Eagle's Minimum Essential Medium (EMEM)
30-2003
Fetal Bovine Serum (FBS)
30-2020
Trypsin-EDTA Solution, 1X
30-2101
Dimethylsulfoxide (DMSO)
4-X
Dulbecco's Phosphate Buffered Saline (D-PBS), 1X
30-2200
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Detailed product information
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General
- Specific applications
-
This cell line is a suitable transfection host.
Characteristics
- Growth properties
- Adherent
- Derivation
- This is one of a number of cell lines derived from malignant gliomas (see alsoATCC®HTB-15™andHTB-16™)by J. Ponten and associates from 1966 to 1969 (see also Allen, 2016).
- Ethnicity
- Unknown
- Gender
- Male
- Karyotype
- This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found.
- Tumorigenic
- Yes;
Yes, in nude mice inoculated subcutaneously with 10(7) cells
- Isoenzymes
-
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 1
PGM1, 2
PGM3, 1
- Comments
-
Mycoplasma contamination was eliminated in September 1975.
The ATCC®HTB-14™cell line was deposited at ATCC in 1982.
STR profiling, Y-chromosome paint, and Q-band assay confirmed that thecell line is male in origin. Based on current literature, the cell line is likely a glioblastoma of CNS origin (Allen, 2016).
Handling information
- Unpacking and storage instructions
-
- Check all containers for leakage or breakage.
- Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
- Complete medium
- The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
- Temperature
-
37°C
- Atmosphere
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95% Air, 5% CO2
- Handling procedure
-
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
- Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
- Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
- Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.
- Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). and dispense into a 25 cm2 or a 75 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
- Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.
- Subculturing procedure
- Volumes used in this protocol are for 75cm2flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
- Remove and discard culture medium.
- Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. - Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting
- Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
- Reagents for cryopreservation
- Culture medium, 95%; DMSO, 5% (ATCC 4-X)
Quality control specifications
- Mycoplasma contamination
- Not detected
- STR profiling
- Amelogenin: X,Y CSF1PO: 10,11 D13S317: 8,11 D16S539: 12 D5S818: 11,12 D7S820: 8,9 TH01: 9.3 TPOX: 8 vWA: 15,17 D3S1358: 16,17 D21S11: 28,32.2 D18S51: 13,14 Penta_E: 7,14 Penta_D: 9,14 D8S1179: 10,11 FGA: 18,24 D19S433: 15,15.2 D2S1338: 20,23
History
- Deposited as
- hom*o sapiens
- Depositors
- J Ponten
- Special collection
-
Human Tumor Cell Bank
- Cross references
-
GenBank E02404 DNA encoding exon K of NAP.
GenBank X06981 Human mRNA fragment for amyloid beta-protein (AP) insertion.
GenBank E02405 DNA encoding PI(the active region that has protease inhibitor activity of NAP).
GenBank E02400 DNA encoding NAP(new senile plaque amyloid precursor protein having protease inhibitor).
GenBank E02402 DNA encoding exon I of NAp(new senile plaque amyloid precursor having protease inhibitor).
GenBank E02401 DNA encoding exon H of NAp(new senile plaque amyloid precursor protein having protease inhibitor).
GenBank E02403 DNA encoding exon J of NAP(new senile plaque amyloid precursor protein having protease inhibitor).
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS
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References
Allen M, et al. Origin of the U87MG glioma cell line: Good news and bad news. Sci. Trans. Med. 8(354): 1-4, 2016.
Clark MJ, et al. U87MG decoded: The genomic sequence of a cytogenetically aberrant human cancer cell line. PLoS Genetics 6 (1) : e1000832, 2010.
Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306
Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744
Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504
Tumors developed within 21 days at 100% frequency (5/5).
References
Curated Citations
Allen M, et al. Origin of the U87MG glioma cell line: Good news and bad news. Sci. Trans. Med. 8(354): 1-4, 2016.
Clark MJ, et al. U87MG decoded: The genomic sequence of a cytogenetically aberrant human cancer cell line. PLoS Genetics 6 (1) : e1000832, 2010.
Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306
Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
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